CRISPR has quickly become one the most important and widely used tools in genetic engineering, thanks in large part to its ease of use and low cost. In this module, students will use CRISPR to modify the expression of LacZ to create an easily recognizable phenotype in bacteria colonies. 

Learning Objectives

  • Students will use CRISPR to modify bacteria.
  • Students will grow the modified bacteria on plates and observe the altered phenotype.