| Researcher: | Institution | Research Focus | Techniques/Instrumentation | Potential Projects | Contact Information |
|---|---|---|---|---|---|
| Fitzkee, Nick PhD | Mississippi State University | Research focuses on understanding the relationship between a protein’s flexibility and its function, and in particular how proteins can use structural disorder to their advantage during catalysis. An additional research focus is understanding protein structure on nanoparticle surfaces. Keywords: NMR biophysics protein biochemistry kinetics surface interactions | Students will be exposed to nuclear magnetic resonance (NMR) spectroscopy, fluorescence, biophysical calorimetry, and spectroscopic approaches to studying protein interactions. In addition, students will learn how to express and purify proteins biochemically. | Email: nfitzkee@chemistry.msstate.edu Office: (662)325-1288 | |
| Thornton, Justin PhD | Mississippi State University | Research focuses on both sides of the host-pathogen interaction by characterizing how the innate immune response functions to limit progression of Streptococcus pneumoniae (pneumococcus) infections and also how virulence mechanisms of the pneumococcus enable it to cause disease. The overall goal of our research is to identify new targets for intervention which will help to decrease the morbidity and mortality associated with this pathogen. | Email: thornton@biology.msstate.edu Office: (662)325-8020 | ||
| Priddy, Lauren PhD | Mississippi State University | My research focuses on developing improved biomaterial and biological treatments for bone injury and disease. In particular, we’re studying alternative antimicrobial therapies for combating Staph infection in bone, and we’re using 3D printing and design to create customized biomedical implants for applications such as bone fixation after traumatic injury. | Email: lbpriddy@abe.msstate.edu Office: (662)325-5988 | ||
| Kaplan, Barbara PhD | Mississippi State University | Research focus is to elucidate the mechanisms by which cannabinoid compounds (i.e., those derived from marijuana) alter immune function. Interested in characterizing the mechanisms by which plant-derived compounds alter T cell function and examining the role of CB1 and CB2 in cannabinoid-mediated suppression of B cell function. Two different model systems are being utilized for these studies. The first model is a mouse-adapted influenza strain, A/PR8/34. The second model is the experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS). | Email: bkaplan@cvm.msstate.edu Office: (662)325-1113 Dr. Kaplan's Faculty Page | ||
| Collins, Galen PhD | Mississippi State University | The Collins lab studies how intracellular proteins are degraded by the ubiquitin-proteasome pathway – the major pathway in removing proteins in eukaryotes. We are interested in how the 26S proteasome degrades proteins, how its activity is regulated, and how it is impaired in diseases. We are particularly interested in its relationship to neurodevelopment and neurodegenerative disorders and cancers. | In our work, we use cell culture, cell viability assays, protein half-life studies, and purified proteins that utilize fundamental cell biology and protein biochemistry methods. | To help understand how a mutation leads to a rare neurodevelopmental disorder, the student-scientist will look at how inflammatory gene expressions using RT-qPCR and western blotting in CRISPR-engineered cell lines. To help screen for protease inhibitors with potential applications to cancer treatment, the student-scientist will assist in purifying proteins and testing the inhibition of our protease using plate-based methods. To help understand the effects of antibiotic use on muscle growth in chickens, the student-scientist will assist in measuring the release of the atypical amino acid 3-methyl-histidine from muscle using amino acid labeling and HPLC techniques. | Email: gac263@msstate.edu Office: (662)325-7940 |
| Feugang, Jean PhD | Mississippi State University | The proposed research project will explore the potential benefits of various nanoparticles, including extracellular vesicles, in developing gamete technologies. It offers unique training opportunities for undergraduate students, involving hands-on experience with living cells and animals. This is particularly advantageous for those pursuing studies in biology, biomedical, or public health disciplines. | We use various animal models for gamete quality investigation with a range of advanced methodologies, including standard (small-scale molecular analyses and bioassays), high-throughput (computer-assisted sperm analysis, omics), and bioimaging (nanotechnology, real-time biophotonics) technologies | 1. Nanotechnology tools in semen analysis 2. Omics analyses of semen quality 3. Spectroscopy and post-collection semen (cryo)preservation | Email: j.feugang@msstate.edu Office: (662)325-7567 Dr. Feugang's Faculty Page |
| Meyer, Florencia PhD | Mississippi State University | Dr. Meyer's research focuses on animal viruses and interactions with the host and other bacteria in the respiratory tract | Email: florencia.meyer@msstate.edu Office: (662)325-2640 Dr. Meyer's Lab Page | ||
| Willett, Kristie PhD | The University of Mississippi | Research Focus: Our research aims to understand molecular mechanisms underlying toxicity and/or shed light on the potential adverse outcomes due contaminant exposure using zebrafish models. For example: 1) Mechanisms of benzo[a]pyrene (BaP) toxicity- The goal of this project is to characterize the transcriptomic and epigenetic changes associated with preconceptional exposure to BaP. Specifically, we are studying the role of the aryl hydrocarbon receptor (AhR) in BaP-induced multigenerational adverse outcomes; and 2) Toxicity of cannabidiol (CBD) and Δ9- tetrahydrocannabinol (THC) - The goal of this project is to understand the relative morphological, neurobehavioral, reproductive and multigenerational phenotypes that result after developmental exposures to CBD and THC. | Email: kwillett@olemiss.edu Office: (662)915-6691 Dr. Willett's Faulty Page | ||
| Laing, Brenton PhD | The University of Mississippi | The Laing Lab focuses on neuronal circuits that control motivated behaviors and physiological changes during interaction with the environment in mice. This includes investigation into fight/flight, feeding, and foraging systems in the brain that exhibited altered regulation during substance dependence and post-traumatic stress disorders. By integrating cutting edge neuroscience tools (optogenetics, chemogenetics, calcium imaging) with classic pharmacological principles and behavioral paradigms the Laing Lab seeks to develop treatment targets that can reduce suffering in humans impacted by these disorders. | Email: btlaing@olemiss.edu Office: (443)668-8498 | ||
| Karim, Shahid PhD | The University of Southern Mississippi | Research focuses on the arthropod vectors of deadly infectious disease agents and attempts to use functional genomics tools to identify tick-derived proteins required for prolonged blood-feeding and pathogen infection in the mammalian host. Keywords: RNA interference, Genomics, proteomics, transcriptomics, recombinant protein expression, vaccine, Alpha-Gal Syndrome, Lyme Disease, Rickettsiosis, ticks, Acari | RNA interference, western blotting, PCR, real time quantitative PCR, confocal imaging, bioinformatics, ELISA, Mass spectrometry, cell culture | Email: shahid.karim@usm.edu Office: (601)266-4748 | |
| Xu, Hao PhD | The University of Southern Mississippi | The Xu lab has three major research focuses: 1) We are interested in understanding specific protein-protein interactions and protein-lipid interactions that lead to membrane fusion, which is required for intracellular traffic, hormone secretion and neurotransmission. To achieve that, we exploit a multifaceted approach which includes biochemical analysis, biophysical measurements and in vitro reconstitution. 2) We are interested in unraveling the regulation of mast cell degranulation, which is the culprit of allergic inflammation. We exploit cell-based secretion assays developed in the Xu lab to interrogate the function of specific proteins in the release of different mast cell mediators, from histamine to TNF. We routinely use RNAi to knock down gene expression or CRISPR technology to knock out a target gene. Our goal is to establish functional assays for proteins that are potential drug targets for the control of allergic inflammation. 3) We are interested in host-viral interactions that play central roles in the survival/propagation of Kaposi’s sarcoma-associated herpesvirus in host cells. At the moment, we are investigating the biochemical and functional relationship between SNAREs and previously overlooked tegument proteins. This line of investigation is conducted in collaboration with colleagues in other universities Keywords: Membrane fusion; secretion; protein traffic; mast cell degranulation; allergy; host-viral interaction | Molecular cloning including PCR, restriction digestion, ligation, DNA isolation; Protein expression and purification; SDS-PAGE; western blotting; preparing proteoliposomes; FRET-based vesicle fusion assay; cell culture; RNAi; CRISPR | The Xu Lab studies mast cell exocytosis, which is key to allergic inflammation and native immunity. An INBRE student will typically work with a graduate researcher to create essential research tools (e.g., generate a DNA construct for CRISPR-mediated gene silencing) | Email: hao.xu@usm.edu Office: (601)266-4748 Dr. Xu's Faculty Page |
| Ashpole, Nicole PhD | University of Mississippi | One of the most debilitating aspects of advanced age is the accompanying cognitive decline. Patients that experience cognitive deficits are faced with the loss of independence, decreased healthspan, and increased financial burden. Studies suggest that age-related cognitive decline is potentially reversible, but the underlying mechanistic changes have yet to be fully elucidated. My research program focuses on identifying how reduced neuroendocrine signaling impacts the function of neurons, astrocytes, and the supporting vasculature in the aging brain. Using genetic and pharmacological tools, we are able to manipulate growth hormone and IGF-1 signaling within these cells and examine how learning and memory is affected. We then examine the ensuing structural, biochemical, and genetic changes within the brain to understand which signaling cascades are contributing to the observed alterations in learning and memory. We are particularly interested in identifying which kinase signaling cascades are altered by the loss of these endocrine signals in advanced age. By characterizing these changes, we hope to uncover pharmacological targets that may delay or avert the onset of age-related cognitive decline. | Email: nmashpol@olemiss.edu Office: (662)915-2407 | ||
| Shawky (Elsayed), Noha M. PhD | University of Mississippi Medical Center | Long-term consequences of hyper-androgenemic pregnancy on offspring | Our research focuses on studying the cardiovascular and renal outcomes in male and female offspring exposed to maternal hyperandrogenemia in-utero. Polycystic ovary syndrome (PCOS) is the most common endocrine disorder and most common cause of hyperandrogenemia in women. PCOS women have difficulty getting pregnant and the long-term health of their offspring is an area that still needs a lot of research. | Email: nelsayed@umc.edu Office: (601)984-1877 | |
| Wallace, Kedra PhD | University of Mississippi Medical Center | Research focuses on the role of T cells in contributing to the breakdown of the blood brain barrier in women with hypertensive pregnancies. Our lab studies both the acute affects and the long-term consequences of BBB damage during pregnancy.The gynecology section of our laboratory examines the relationship between hypertension and uterine fibroid smooth muscle cell proliferation. We also examine how women’s health conditions such as uterine fibroids and endometriosis affect quality of life, depression and anxiety among women with these disorders. Keywords: Women’s health, hypertension, mental health, neuroscience, obstetrics, gynecology | Students rotating through my lab may participate in a variety of techniques ranging from rodent behavior to brain cellular dissection to flow cytometry preparation to the more common enzyme and protein based assays. | Email: kwallace2@umc.edu Office: (601)984-5396 Dr. Wallace's Faculty Page | |
| Tucci, Michelle PhD | University of Mississippi Medical Center | Email: mtucci@umc.edu Office: (601)815-1043 Dr. Tucci's Faculty Page | |||
| Duggar, W. Neil PhD | University of Mississippi Medical Center | Dr. Duggar research interests are in clinically applied research in the field of Radiation Oncology. This may include the handling of CT, MRI, PET, or other patient imaging as well as the harvesting of dosimetric data from patient treatment plans treated with external beam radiation therapy, Gamma Knife, or even High-Dose Rate Brachytherapy. Huge interest lies in the application of big data, artificial intelligence, and machine learning technologies to further clinical quality at the University of MS Medical Center, though this is a developing infrastructure. Keywords: Radiation Oncology, Machine Learning, DICOM, Radiosurgery | The student will be exposed to many aspects of Radiation Therapy such as treatment planning systems, linear accelerators, Gamma Knife Icon, HDR Afterloaders. | My research tends to be related to clinical application of new technology related to radiation therapy practice. This may include software solutions for patient management, implementation of Artificial Intelligence, or even adoption of new treatment planning methodologies. Additionally, I have some work going on with AI development for clinical applications such as ECE identification in head and neck patients. | Email: wduggar@umc.edu Office: (601)815-6246 |
| Warrington, Julie P. PhD | University of Mississippi Medical Center | Research focus is on hypertensive disorders of pregnancy and neurovascular and cognitive function in the mom and offspring. My lab also studies mechanisms for seizures in pregnancy. | nockout and reporter mice), immunofluorescence staining, molecular and cellular approaches, and behavioral testing. We have access to a Laser Speckle Imager (measuring cerebral blood perfusion), blood gas analyzer, fluorescence microscope, Power Lab (blood pressure measurement), confocal microscope (in colleague’s lab), and other equipment for Western blots, ELISA, etc…. | Email: jpwarrington@umc.edu Office: (601)815-8969 Dr. Warrington's Faculty Page | |
| Li, Ling | Mississippi State University | My primary scientific goals focus on advancing our understanding of plant metabolism and adaptation, with direct applications in agriculture. My work aims to contribute to sustainable agricultural practices by integrating experimental research and computational biology. | Extraction of DNA/RNA/Protein, Gel electrophoresis, PCR, Real-time PCR, Starch staining and quantification, Protein quantification, Molecular cloning | The student would work on projects focused on enhancing crop resilience and productivity using molecular biology and bioinformatics approaches. This includes studying the regulatory networks that control plant metabolism and adaptation, as well as manipulating orphan genes like the Arabidopsis QQS gene to improve protein content and stress tolerance in various crops. The student would have the opportunity to apply these findings to agricultural biotechnology, contributing to the development of sustainable crop varieties. | Email: liling@biology.msstate.edu Office: (662)325-7570 Dr. Li's Faculty Page |
| Li, Ji PhD | University of Mississippi Medical Center | Dr. Ji Li’s Lab primarily seeks to understand the intrinsic relationship between diabetes, cardiovascular diseases, and neurodegenerative disease. Evidence suggests that adenosine monophosphate-activated protein kinase (AMPK) is a critical metabolic sensor to maintain metabolic homeostasis and inflammatory response under physiological and pathological conditions. Dr. Li’s group wants to elucidate the molecular mechanisms responsible for AMPK activation, identify novel downstream AMPK targets, and develop therapeutic techniques that target the enzyme to prevent and treat myocardial ischemia, hypertension, cardiac hypertrophy, diabetes, and Alzheimer’s disease related dementia. | Surgical approaches to generate animal models of myocardial infarction, heart failure, cardiorenal syndrome, traumatic brain injury. Echocardiography measures cardiac systolic and diastolic functions under physiological and pathological conditions. Quantitative electroencephalography for measurement of traumatic brain injury, epilepsy, and Alzheimer’s disease related dementia. A working heart perfusion system with radioactive glucose and oleate to determine cardiac metabolism under physiological and pathological conditions. IonOptix myocyte calcium and contractility systems to determine contractile functions of cardiomyocytes in response to pathological challenge. Seahorse XF Cell Mito Stress Test measures alterations in mitochondrial energy metabolism under physiological or pathological conditions. 10x Genomics Single Cell Technique and common biochemical/molecular analysis investigate the signal transduction of heart and brain in response to pathological challenge in aging. | Email: jli3@umc.edu Office: (601)815-8995 Lab: (601)984-1839 Dr. Li's Faculty Page | |
| Raucher, Drazen PhD | University of Mississippi Medical Center | Research focuses on developing an approach that allows peptide therapeutics to be delievered specifically to the tumor site. | One focuses on drug delivery of therapeutic peptide and small molecule drugs in cancer research. Another involves testing natural products for anticancer activity. The third is centered on testing a small molecule in vivo in Huntington’s disease genetically modified mice. | Email: draucher@umc.edu Office: (601)984-1510 Dr. Raucher's Faculty Page | |
| Drummond, Heather PhD | University of Mississippi Medical Center | Research focuses on identifying proteins that form mechanosensitive ion channels in sensory neurons and vascular smooth muscle, understanding how mechanosensitive channels transduce force, and seeing how these channels are affected by disease states such as hypertension. | Techniques used in the lab include cell culture (neurons, macrophages, smooth muscle cells), molecular biology (PCR, western blotting, immunofluorescence) , flow cytometry of hypothalamic neurons, and genetically modified mouse models | Mechanism of arcuate neuron degenerin ion channels in protection against obesity and maintenance of metabolic homeostasis. Regulation of myogenic tone and susceptibility to hypertensive renal injury Mechanisms regulating macrophage polarization. Inflammatory cells, vascular injury and renal disease. | Email: hdrummond@umc.edu Office: (601)984-1812 Dr. Drummond's Faculty Page |
| Janorkar, Amol PhD | University of Mississippi Medical Center | With his training and experience in the field of biomaterials and tissue engineering over the past 19 years, Dr. Amol Janorkar leads a research group that focuses on cell-biomaterial interactions to direct cell morphology and ultimate cell function. The Janorkar Lab uses chemical and physical modification of biopolymer substrates to create three-dimensional in vitro tissue models that achieve enhanced survival and biological function versus conventional cultures for liver, adipose, and bone tissue engineering. With his research funded by the NIH, NSF, and USDA, Dr. Janorkar has published 27 journal articles and 27 conference proceedings, and made 44 conference presentations in the past 5 years. Recognizing these research accomplishments the University of Mississippi Medical Center has awarded Dr. Janorkar the Gold, Silver, and Bronze Medallions for Research Excellence. Dr. Janorkar serves as the Director of the summer research program that has trained 140+ dental and undergraduate students over past 11 years. Recognizing his contributions to dental research, he has been inducted into the Omicron Kappa Upsilon National Dental Honor Society, which rarely inducts non-dentist faculty members. Dr. Janorkar has supervised graduate (6), undergraduate (32), dental and medical (10), and post-docs (2). His students have won 32 awards for outstanding research at local and national levels. Recognizing his teaching and mentoring, Dr. Janorkar was awarded the TEACH (Toward Educational Advancement in Care and Health) Prize, the highest award given to an educator by the University of Mississippi Medical Center. He has also been inducted into the Nelson Order of Teaching Excellence. Keywords: Tissue engineering, Drug delivery, Polymeric biomaterials | Materials synthesis and characterization, Cell culture, Biological responses | Student will focus on understanding cell-biomaterial interactions to direct cell morphology and enhance cell function. They will use chemical and physical modification of biopolymer substrates to create three-dimensional in vitro tissue models for tissue engineering. They will also prepare micro- and nano-particles and hydrogels to achieve controlled and long-term drug delivery. | Email: ajanorkar@umc.edu Office: (601)984-6170 Dr. Janorkar's Faculty Page |
| Fan, Lir-Wan PhD | University of Mississippi Medical Center | Research focused on investigating the long-term adverse effects of perinatal brain inflammation on late-onset neurodegenerative diseases, such as idiopathic Parkinson’s disease. Studies: Perinatal hypoxic‐ischemia, Periventricular leukomalacia, Intrauterine growth restriction, Parkinson’s disease, Inflammatory and neuropathic pain, Sleep disturbances. Keywords: Neurotoxicity; Development, Inflammation, Cytokines, Neonate, Hypoxic‐ischemia, Neurodegenerative diseases, Neuronal behavior, Brain damage, Spinal cord injury, Motor dysfunction, Cognitive deficits, Inflammatory and neuropathic pain. | Animal behavioral study, sample collection, ELISA, Western blotting, TBARS assay, enzyme activity assay, ATP assay, fixation, microtomy, immunohistochemistry, microscopy, stereological cell counting, IVIS spectrum imaging, micro-CT imaging, unilateral or bilateral carotid artery occlusion surgery, reduced uterine perfusion surgery, stereotaxic techniques, Microlight (ML830) Laser therapy, and transcutaneous electric nerve stimulation (TENS) therapy. | One to three sentences on what sort of projects the student would working on: My research is to investigate whether perinatal brain inflammation and intrauterine growth restriction (IUGR) contribute to long-term effects and the treatment of maternal or neonatal inflammation-induced Attention-deficit/hyperactivity disorder (ADHD) and white matter disease using inflammation (LPS)-induced Periventricular leukomalacia (PVL) model. In addition, we also test and optimize a hydrogel matrix to improve the calvarium's ossification rate in the craniectomy area and preserve the neural architecture and function in the underlying brain parenchyma in young animals with traumatic brain injury (TBI). | Email: lwfan@umc.edu Office: (601)984-2345 Dr. Fan's Faculty Page |
| Stray, Stephen PhD | University of Mississippi Medical Center | Research focuses on virus evolution, using influenza virus as a model, and developing virus-based therapeutics for the treatment of cancer Keywords: influenza, virus evolution, cancer therapy, protein purification, virus assembly | Flu project: computational analysis of databases, classic virology techniques (hemagglutination, virus growth, plaque reduction assay, chemical neuraminidase assay), in vitro virus evolution. Cancer therapy project: cell growth retardation assays, cytotoxicity assays, cell cycle analysis, bacterial protein expression and purification, in vitro virus-like particle assembly, mass spectrometry. | Email: sstray@umc.edu Office: (601)984-1735 Dr. Stray's Faculty Page | |
| Vidal, Jorge E. PhD | University of Mississippi Medical Center | Molecular pathogenesis of Streptococcus pneumoniae (pneumococcus). Mechanism(s) of colonization of the upper airways. Molecular epidemiology of pneumococcal disease. Acquisition of antibiotic resistance. Keywords: Bacterial infections, pneumococcal disease, bacterial pathogenesis, bacterial genetics, antibiotic resistance, vaccines | PCR and Real-time PCR assays Protein electrophoresis and Western-blot TaqMan array cards Bacterial cultures and Cell cultures Fluorescence microscopy and confocal microscopy Gene expression studies, RT-PCR, and RNA-Seq Serology DNA and RNA purification Cellular fractionation | Email: jvidal@umc.edu Office: (601)815-1236 Dr. Vidal's Lab Page | |
| Taylor, Erin PhD | University of Mississippi Medical Center | Immunology, cardiovascular and renal physiology | Email: ertaylor@umc.edu Office: (601)984-1842 Dr. Taylor's Faculty Page | ||
| Gibert, Yann PhD | University of Mississippi Medical Center | My overall goal is to use the zebrafish as a vertebrate organism to develop models of endocrine disrupters action, to model metabolic disorders and to study Hem/Onc using different zebrafish models and test novel putative therapeutics to fight these diseases. | CRISPR/cas9, in situ hybridization, omics, developmental biology | Using Zebrafish to model fetal hyperglycemia: Using glucose exposure and different CRISPR-cas9 mutant my lab investigates the molecular consequences of fetal hyperglycemia Using zebrafish models of xenograft using human cancer cells my lab test novel putative chemotherapeutics in vivo AML (Acute Myeloid Leukemia) studies in Zebrafish: My lab has a humanized zebrafish model of AML. We are using this model to test novel therapeutics in vivo and understand their mode of action | Email: ygibert@umc.edu Office: (601)815-1659 Dr. Gibert's Faculty Page |
| Singh, Ajay PhD | University of Mississippi Medical Center | Dr. Singh’s laboratory focuses on the identification of novel tumor-associated genes and how these genes influence the behavior of tumor cells making them more aggressive. His lab also focusses on understanding how the tumor cells communicate with the host cells and adapt to the harsh and challenging surrounding environment, and if targeting of these interactions could be exploited for cancer prevention and therapy. | We use several biochemical and molecular biological techniques such as semi-quantitative and quantitative polymerase chain reaction (PCR), western blotting, immunofluorescence, immunohistochemistry, genetic engineering, site-directed mutagenesis, cell culture, transient and stable transfections, promoter-reporter assays, ELISA, etc. | - Study the effect of nicotine exposure of prostate tumor biology. - Examine the expression of certain proteins involved in prostate cancer biology and therapy resistance in patient tumor samples - Study the effect of stress hormone, inflammatory cytokines, and nicotine on gene expression in prostate cancer cell lines and delineate underlying molecular mechanisms. | Email: asingh1@umc.edu Office: (601)815-6850 Lab: (601)815-8309 Dr. Singh's Faculty Page |
| Butler, Kenneth PhD | University of Mississippi Medical Center | Email: kbutler@umc.edu Office: (601)984-4939 | |||
| Camlin, Nicole PhD | University of Southern Mississippi | Research in my lab investigates how phosphatases regulate cell signaling pathways, with a specific interest in M-Phase of meiosis and mitosis. Importantly, errors in M-Phase can lead to trisomy, infertility, and miscarriage (meiosis) and cancer (mitosis). My research is particularly interested in M-Phase of oocyte meiosis; however, future research plans to extend our meiotic research into mitosis. | Mammalian cell culture (primary oocyte cells), standard molecular biology techniques (e.g., immunoblotting, PCR, molecular cloning), advanced microscopy and micromanipulation, standard yeast molecular and cellular biology techniques (e.g., growth assays, gene editing). | It is widely accepted that PP1 is essential for normal M-Phase progression in mitosis and oocyte meiosis; however, the contribution of PP1 polymorphisms to health and disease is unclear. Projects in the Camlin lab will utilize “humanized yeast” to answer important questions about the role of cancer-associated PP1 polymorphisms for M-Phase timely and accurate M-Phase progression. Experiments will include molecular cloning, yeast cell culture, fluorescent microscopy, data analysis, and statistical analysis. | Email: Nicole.Camlin@usm.edu Dr. Camlin's Lab Page |
| Keller, Lance PhD | University of Mississippi Medical Center | Research focuses on host-pathogen interactions of Streptococcus pneumoniae and the human host. The lab is interested in identifying new bacterial processes that are utilized to overcome the innate immune system and allow development of serious disease. Also, how chronic inflammation impacts pneumococcal disease or particular interest, specifically inflammation caused by microplastic exposure and obesity. | Molecular cloning techniques, animal models of infection, immunological profiling with flow cytometry, cell culture models, and different microscopy techniques. | Email: lekeller@umc.edu Office: (601)984-1914 Dr. Keller's Lab Page | |
| Oyugi, Daniel PhD | Mississippi Valley State University | My research focuses on examining the underlying cellular and molecular mechanism(s) by which Vernonia amygdalina (VA) leaf extracts inhibit growth and proliferation of cancer cells, in vitro. We aim to uncover if extract fractions disrupt microtubule organization causing changes that mediate cell cycle arrest and trigger apoptosis. Further, we aim to explore VA activities on mitochondrial and cellular metabolic processes in cancer cells, as well as monitor gene and protein expression profiles of Multidrug Resistance Proteins in those treated cells. | "Techniques 1. Viability assay 2. Apoptosis assays 3. Immunofluorescence 4. Tubulin turbidimetry 5. Flow cytometry 6. Polymerase Chain Reaction (PCR) 7. Agarose Gel Electrophoresis 8. Bioenergetics assay- Seahorse XFe96 analyzer Equipment 9. Mammalian Cell culture incubator 10. Biotek Microplate Reader 11. Gel imaging equipment 12. Table-top centrifuges 13. Liquid Nitrogen cryogenic tank 14. Ultra-low freezer (-80 ᵒC) 15. Spectrophotometer 16. Water bath 17. Gel Transilluminator 18. Epifluorescence microscope 19. Gel electrophoresis equipment 20. Fluorescent Microscope- available at an external lab 21. FACS-Flow Cytometry – available at an external lab 22. Seahorse XFe96 Flux Analyzer- available at an external lab 23. Gas Chromatography-Mass Spectrometry (GC-MS)- available at an external lab" | "Student 1 will assess potential effects of Vernonia amygdalina (VA) fractions on mitochondrial and membrane function in cancer cells The student will apply biochemical techniques to examine if VA inhibits viability in cancer cells. Specifically, MTT assay- a test for mitochondrial and cell membrane function, as well as other quantitative or calorimetric assays, will be used to determine cytotoxicity, growth inhibition and anti-proliferative activities of VA fractions in cells. Absorbance will be measured on a Biotek microplate reader. Results will be analyzed using GraphPad Prism (statistics software). FACS (flow cytometry) will augment MTT assay data, as well as measure DNA content and detect cell cycle arrest and apoptosis. Further, tests for DNA damage will be conducted by measuring activity of damage-associated enzymes such as Poly (ADP) ribose polymerase (PARP). Through this assay, single-strand DNA breaks (SSB) can be detected. Use of TUNEL assay and Double Stain Apoptosis Detection kit will also help monitor apoptosis. Student 2 will determine if VA fractions disrupt microtubule organization in cancer cells The student will apply immunofluorescence techniques to examine if microtubules in treated cells undergo assembly or disassembly. Immunocytochemical evaluation will involve use of antibodies specific for alpha and beta tubulin in conjunction with a secondary antibody that is conjugated to a fluorophore such as phalloidin. Changes in the distribution, architecture and organization of microtubule protofilaments will be examined using a Fluorescence microscope. Additionally, analysis of polymerization or depolymerization of microtubules will be conducted either by florescence microscopy or tubidometric assay, or by both. Also, application of Fluorescence microscopy will help detect morphologic hallmarks of apoptosis. We will examine how changes in microtubule organization mediate cell cycle arrest and trigger apoptosis. " | Email: daniel.oyugi@mvsu.edu |
| Siddiqui, Jawed PhD | University of Mississippi Medical Center | Dr. Siddiqui is an Assistant Professor in the Department of Cell and Molecular Biology at the University of Mississippi Medical Center. His lab focuses on the biology of chemokines and growth factors in the context of bone remodeling and metastasis. Bone metastases or dissemination of cancer cells within the bone are considered an advanced, debilitating, and incurable disease. Bone metastasis is common in prostate cancers, which is the second leading cause of cancer-related deaths among men in the U.S. Unfortunately, current therapeutic targeting of bone metastasis remains palliative due to unclear mechanisms and lack of druggable targets. In addition, most of the traditional therapies often fall short due to the complex bone microenvironment, which fosters tumor growth and resistance to treatment.One of the major objectives of Dr. Siddiqui’s laboratory is to elucidate biological mechanisms underlying bone metastasis of different malignancies such as prostate, breast, and lung cancers. He has developed unique techniques and experimental mouse models to understand the mechanism of bone metastasis and therapeutics. Using the in-vivo and in-vitro technique, his lab is interested in dissecting how metastatic cancers evolve to manipulate the immune system dysfunction and the role of Osteomacs (bone-residing macrophages) in primary and metastatic sites among tumors of varied genetic backgrounds. If you are enthusiastic about advancing the research on bone metastasis, join our collaborative team. We always look for a highly enthusiastic researcher to join as graduate students, summer fellows, and clinical fellows. We welcome opportunities for collaboration. | Dr. Siddiqui's lab is dedicated to advancing the understanding of bone metastasis in cancer to address how cancer spreads to the bones and affects patient outcomes. Our lab employs state-of-the-art techniques to explore the complex interaction between cancer cells and the bone microenvironment. Students will be able to engage in hands-on training with advanced methodologies, including in vitromodels, imaging, and molecular biology techniques. By participating in this research, students will contribute to understanding the metastasis research that could lead to novel therapeutics. | Email: jsiddiqui@umc.edu Office: (601)815-0592 Dr. Siddiqui's Faculty Page | |
| Rezq, Sumar PhD | University of Mississippi Medical Center | My research focuses on studying mechanisms that are involved in kidney injury in Polycystic Ovary Syndrome and their modulation by the diet. | ELISA, Colorimetric assays, Microscopy, cell culture techniques, Western Blot, qPCR, etc | Students will be working on studying mechanisms that are involved in kidney injury in Polycystic Ovary Syndrome. The project involves cell culture studies on kidney cells, as well as assessing some markers related to the kidney pathology in samples that were collected from research animals. | Email: srezq@umc.edu Office Phone: 601-984-1852 Dr. Samar Rezq's Faculty Page |
| Butler, Kenneth PhD | University of Mississippi Medical Center | My work is involves the histology and microscopy core in the Department of Pharmacology and Toxicology at UMMC. My research focuses on developing methods to best analyze cells and tissues using a variety of staining, imaging, and analysis techniques. My lab is also a resource for other colleagues in the department with imaging and analysis needs on their projects. | "Multiple brightfield and inverted microscopes for studying cells and tissues Nikon digital cameras for capturing cell and tissue images Nikon Image analysis software Routine, special, immunohistochemical, fluorescent staining techniques" | Students could be involved in any number of projects that include analysis of cells and tissues (like the pancreas and kidney) from a variety of experimental models treated with novel compounds. These projects would involve determining differences in cell populations and tissue structure between those treated and the control group. In addition, they would have the opportunity to compare other lab data from serum studies, like inflammation levels, on cell and tissue morphology. | Email: kbutler@umc.edu Office Phone: 601-815-9453 Dr. Kenneth Butler's Lab Page |
| Heda, Ghanshyam, PhD | Mississippi University for Women | My laboratory has recently identified few small molecule compounds (triazole derivatives) that can increase the expression of intracellular mutated CFTR protein. One of our current goals, therefore, is to identify biochemical mechanisms that can help traffic these newly upregulated intracellular mutated-CFTR protein from intracellular compartments to the cell surface and make it functional | My research laboratory at MUW is equipped with modern instruments necessary for the detection of protein expression by western blotting and gene transcription by RT-qPCR techniques. | My laboratory engages with various projects related to cystic fibrosis. More specifically we analyze the ability of small synthetic molecules in upregulation of CF causing mutated protein called CFTR. My laboratory also works on determining the mechanisms of rapid degradation of most common CFTR mutation DF508. | gdheda@muw.edu |
| Salazar Marocho, Susana DDS, MS, PhD | University of Mississippi Medical Center | Use of biowaste for developing sustainable biomaterials. Novel surface modifications on high-strength biomaterials for enhanced performance | Dr. Salazar Marocho employs a variety of advanced techniques in her research, including bond strength testing, thermomechanical testing, and mechanical testing of dental materials. Her work often utilizes scanning electron microscopy, energy dispersive spectroscopy, 3D surface profiler, plasma-gas surface deposition, air particle abrasion, along with techniques for assessing long-term performance (fatigue testing) and biocompatibility of biomaterials. | Dr. Salazar Marocho focuses on the design, testing, and characterization of dental ceramics, polymers, and composites, focusing on improving mechanical properties, durability, and biocompatibility. Her research includes assessing bonding mechanisms, thermoresponsive polymers, fracture behavior, and long-term performance. She is also exploring the use of biowaste for developing sustainable biomaterials. | Email: ssalazarmarocho@umc.edu Office: 601-984-6170 |
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