Recombinant DNA Technologies

Students will be introduced to genetic engineering using the isolation, purification, and amplification of specific DNA fragments. Molecular cloning will be performed with the Gibson Assembly Method. Green-fluorescent protein (gfp) will be cloned into plasmid and transformed into Escherichia coli.

Learning Objectives

Students will learn to set up Polymerase Chain Reaction (PCR).
Students will learn to run agarose gel electrophoresis.
Students will learn to purify PCR products.
Students will learn to use Isothermal (Gibson) assembly cloning.
Students will learn to transform Escherichia coli.
Students will learn to isolate plasmid DNA.

DannyHolland2023-03-21T13:32:17-05:00March 13th, 2023|

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Mississippi INBRE
The University of Southern Mississippi
118 College Drive #5018
Hattiesburg, MS 39406

msinbre@usm.edu

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“The Mississippi INBRE program is supported by Award Number P20GM103476 from the National Institutes of General Medical Sciences. The content of this website is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of General Medical Sciences or the National Institutes of Health.”