The Mississippi INBRE Cell Bioenergetics Facility, hosted by the Mississippi Nano-Bio and ImmunoEngineering Consortium (NIEC) at the University of Mississippi, functions as a dynamic hub driving innovation in biomaterial and biomolecular research across the state. The Cell Bioenergetics facility gives access to a multitude of cutting-edge instruments, equipping researchers with the tools necessary to achieve groundbreaking advancements in biomedical research. These resources are available to a diverse range of individuals, spanning from faculty to ambitious undergraduates alike. Importantly, the Cell Bioenergetics Facility aims to serve as a bridge between technological advancement and scientific ingenuity, fostering an environment dedicated to building a better Mississippi. Equipment available through the facility enable in vivo imaging, cell microscopy, flow cytometry and cell sorting, traditional biomolecular analysis and single cell assays.
Contact Dr. Thomas Werfel at tawerfel@olemiss.edu or 662.915.3126 for more information.
Equipment List
| Image | Name | Description |
|---|---|---|
 | IVIS Lumina Series III | The IVIS Lumina Series III is a state-of-the-art optical imaging system for non-invasive in vivo and in vitro studies. It employs bioluminescence and fluorescence imaging to monitor gene expression, molecular pathways, and cell tracking. With a sensitive CCD camera, it quantifies signals accurately and allows multi-fluorophore imaging. Valuable for preclinical research, it provides insights into disease models, drug effects, and therapeutic responses, reducing the need for invasive sampling. |
 | Bigfoot Spectral Cell Sorter | The Bigfoot Spectral Cell Sorter employs cutting-edge spectral cytometry technology, integrating laser excitation and advanced wavelength analysis to segregate cells. This methodology precisely identifies cells through their distinct fluorescent markers, enabling multiparametric characterization. The subsequent droplet-based sorting employs intricate hydrodynamic forces to isolate specific cell populations with exceptional precision, making it invaluable for genomics and immunophenotyping studies. |
 | Attune NxT Flow Cytometer | The Attune NxT Flow Cytometer employs innovative hydrodynamic focusing and laser-induced fluorescence excitation techniques. This enables the analysis of cells at the individual level, yielding valuable fluorescence emission and scatter data for detailed cellular attribute assessment. This high-speed cytometer excels in applications such as cell cycle analysis, apoptosis studies, and identification of rare cell subsets, offering in-depth and accurate cellular characterization. |
 | Cytation C10 Confocal Imaging Reader w/ BioSpa 8 Automated Incubator | The Cytation C10 Confocal Imaging Reader combined with the BioSpa 8 Automated Incubator offers enhanced live-cell imaging and analysis. The confocal imaging system provides high-resolution images by eliminating out-of-focus light through laser scanning. Its spectral detection capability enables multiplexed labeling experiments. The BioSpa 8 Incubator maintains optimal conditions for extended live-cell studies. This duo is crucial for observing dynamic cellular processes and interactions in real time. |
 | Cytation 5 Cell Imaging Multi-Mode Reader | The Cytation 5 Cell Imaging Multi-Mode Reader uniquely integrates advanced microscopy and multi-mode detection capabilities. Its microscopy module captures high-resolution images of multiwell plates, enabling precise cell counting and comprehensive morphological analyses. The multi-mode detection unit combines fluorescence, luminescence, and absorbance measurements in a single platform, facilitating diverse high-throughput cellular assays with unprecedented versatility. |
 | Lionheart LX Automated Microscope | The Lionheart LX Automated Microscope integrates precision optics with motorized stage control and customizable automation protocols. The optical system offers brightfield and fluorescence imaging modalities with adjustable illumination and filters. Combining cutting-edge mechanics and electronics, this system automates imaging processes, minimizing user intervention for live-cell imaging, kinetic assays, and detailed image-based studies. |
 | Seahorse XFe96 Analyzer | The Seahorse XFe96 Analyzer is an progressive instrument used to study cellular metabolism. It employs real-time, label-free analysis in a microplate format to assess mitochondrial function and energy dynamics. By precisely controlling oxygen and pH levels, it monitors oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), unveiling cellular respiration and glycolysis. Chemical probes enable dynamic measurements of metabolic parameters, making it essential for diverse research like cancer biology, immunology, and drug discovery. |
 | iBright CL750 Imaging System | The iBright CL750 Imaging System merges chemiluminescence and fluorescence imaging capabilities with a high-sensitivity camera. This combination offers optimal signal detection and quantification. With precise temperature control and specialized filters, the system delivers high-resolution images critical for accurate protein analysis, western blotting, and gel documentation. Its versatile software enables quantitative analysis and adaptability to various research needs. |
 | QuantStudio Absolute Q Digital PCR System | The QuantStudio Absolute Q Digital PCR System employs microfluidic arrays and thermal cycling control to divide samples into a multitude of reaction wells. This advanced thermal and fluidic management ensures precise temperature uniformity, enabling digital PCR reactions with high precision. The microfluidic array design guarantees single-molecule partitioning, essential for absolute nucleic acid quantification and rare variant detection. |
 | Chromium iX Single Cell System | The Chromium iX Single Cell System leverages a microfluidic chip that encapsulates single cells with barcoded beads. The precisely engineered oil-coated microfluidic channels create aqueous droplets containing individual cells and barcoded beads. This method enables high-throughput, single-cell genomics by capturing cells and barcodes in separate compartments, subsequently enabling multi-omics profiling. |
 | 4150 TapeStation System | The 4150 TapeStation System revolutionizes nucleic acid and protein analysis with automated capillary electrophoresis. Its microfluidic chip integrates separation, detection, and quantification. The precisely controlled electric fields enable rapid sample separation in gel-like matrices. The system's software translates migration rates into quality metrics, facilitating automated sample quality assessment, sizing, and quantification. |
 | QuantStudio 3 Real-Time PCR System | The QuantStudio 3 Real-Time PCR System employs cutting-edge optical technology for fluorescence detection during PCR cycling. The system's advanced optics and thermal management enable real-time monitoring of amplification kinetics. Its software analyzes raw fluorescence data to derive quantitative information. The instrument's rapid cycling capabilities and sophisticated algorithms support various quantitative PCR applications. |
 | SimpliAmp Thermal Cycler | The SimpliAmp Thermal Cycler utilizes leading-edge technology for accurate and rapid temperature control. The thermoelectric module rapidly alternates between temperature setpoints, enabling precise PCR cycling. Its intuitive interface allows users to design and execute temperature profiles with ease, supporting diverse PCR applications, from routine to complex gene expression studies. |
 | Qubit Flex Fluorometer | The Qubit Flex Fluorometer employs targeted fluorometric assays with precise excitation and emission wavelengths. It selectively measures fluorescent signals from DNA, RNA, and proteins, effectively eliminating background noise. Its proprietary dye-based quantification system ensures high sensitivity and broad dynamic range, offering accurate quantification across a range of concentrations. |
 | Countess II Automated Cell Counter | The Countess II Automated Cell Counter leverages sophisticated brightfield imaging and proprietary image analysis algorithms. Its integrated optical system captures high-resolution images of cells, and the algorithmic processing swiftly detects and quantifies cells, along with assessing their viability and size distribution. Automation reduces user variability, ensuring consistent and reliable cell culture analysis. |
 | Sorvall Legend Micro 21R Microcentrifuge | The Sorvall Legend Micro 21R Microcentrifuge combines intricate rotor technology with precise motor control for optimal sample separation. The brushless induction drive motor offers high acceleration and deceleration rates, ensuring rapid and consistent sample processing. Its temperature control and safety features enable reproducible results while minimizing sample degradation. |
 | Sorvall X Pro Series Centrifuge | The Sorvall X Pro Series Centrifuge integrates a powerful drive system with an intelligent user interface. This innovative control system optimizes rotor acceleration, resulting in efficient sample separation. Its adaptive control algorithms cater to various experimental scenarios, ensuring safe and reproducible results across diverse sample types and sizes. |